20100413

speculation

Viral gene therapy : three problems

-- immune response to viral vehicle
-- -- temporarily suppress immune system during treatment ?

-- carcinogenic effect
-- -- include tumor suppressor genes in construct
-- -- include system that can detect "bad" insertion points and
-- -- -- kill cell
-- -- -- fix cell
-- -- -- undo insertion

-- transmission to germ line
-- -- "oops" haha !

-- weird disruptions not otherwise specified (induced metabolic disorder )


-- -- I don't think this has been observed


-- assume insertion into only "open" genes
-- ignore genes that function adequately with a single copy ( read : all the ones that don't have to do with cancer )
-- include extra copies of these genes in the construct ?
-- -- might kill cells by increasing apoptosis / slowing division ?
-- -- might force the construct to be too large
-- -- might not need to supplement _all_ potentially disrupted genes, there are multiple fail-safes

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